Conference Venue: University of Abertay, Dundee Scottish Branch
From: 12 Mar 2008 To: 12 Mar 2008
The effects of 8-OH-DPAT (a serotonin agonist) on light induced phase shifts in mice lacking the 5-HT7 receptor
University of Glasgow
Objectives: To determine more clearly what receptor (s) mediates the effect of 8-OH-DPAT on light induced advances in mice.
Design: Administration of Saline & Light at CT0 causes a phase advance in 5-HT7KO and WT mice which is possibly mediated through the 5-HT1A or 5-HT7 receptor. Using 8-OH-DPAT as a 5-HT1A/7 agonist the receptor subtype (s) mediating this shift should become more clear.
Methods: Within-subjects design. 11 WT, 10 5-HT7KO mice used, maintained in a light/dark cycle to ensure a predictable activity cycle. Then placed in darkness for 10 days prior to treatment with either 8-OH-DPAT and light or saline and light at CT0. Onset of activity for each animal calculated 10 days after treatment.
Results: ANOVA showed a significant interaction between treatment and species and also a significant difference between the 5-HT7KO and WT mice treated with 8-OH-DPAT.
Conclusions: Mice possibly more variable when genetically modified and treated with serotonin. Therapeutic potential for depression.